Dissident AIDS Database

HIVIsolationAntigen testUncorrelation with viral load tests
Direct measurement of viraemia in patients infected with HIV-1 and its relationship to disease progression and zidovudine therapy.
 Semple M et al.
  “there were 16 sera from 30 viraemic patients which did not have detectable p24 antigen. As a consequence, p24 antigen concentration and HIV-1 RNA did not correlate well.”
  J Med Virol. 1991;35:38-45.1991
Diagnosis of primary HIV-1 infection.
 Daar ES, Little S, Pitt J, Santangelo J, Ho P, Harawa N, Kerndt P, Glorgi JV, Bai J, Gaut P, Richman DD, Mandel S, Nichols S
  The accuracy of p24 antigen testing was called into question : some people in the study cohorts were found to be positive for HIV antibodies on initial screening, and were described as having "chronic HIV-infection". The large majority of these people (82%) were negative for p24 antigen, which is a protein thought to be a specific and integral part of the virus. People with viral loads at least as high as 631,000 copies/mL were still negative for p24 antigen, which again raises the question of how much virus was really present in these people.
  Ann Intern Med, January 2, 2001;134(1):25-92001
False-positive HIV-1 virus cultures using whole blood.
 Schupbach, J., Jendis, J. B., Bron, C., Boni, J. & Tomasik, Z.
  The whole blood cultures of 49/60 (82%) of "presumably uninfected but serologically indeterminate" individuals and 5/5 seronegative blood donors" were found positive for p24
  AIDS 6:1545-1546. 19921992
Genomic instability and AIDS.
 Kozhemiakin, L. A. & Bondarenko, I. G.
  The "HIV proteins (p17, p24)" appear in the blood of patients (previously negative for all HIV markers) following "transfusions of HIV-negative blood and UV-irradiation of the autoblood"
  Biochimiia 57:1417-1426. , 1992.1992
False-positive human immunodeficiency virus testing in patients with lupus erythematosus.
 Barthel HR, Wallace DJ.
  p24 is detected in a significant number (up to 36% of patients with systemic lupus erythematosus)
  Semin Arthritis Rheum 1993 Aug;23(1):1-71993
False-positive neutralizable HIV antigens detected in organ transplant recipients.
 Vincent, F., Belec, L., Glotz, D., Menoyo-Calonge, V., Dubost, A. & Bariety, J
  Detection of p24 has been also reported in organ transplant recipients. In one kidney recipient (the donor was negative for p24 antigen) who, 3 days following transplantation developed fever, weakness, myalgias, cough and diarrhoea, all "Bacteriological, parasitological and virological samples remained negative [including HIV PCR]. The only positive result was antigenaemia p24, positive with Abbot antigen kits in very high titers of 1000pg/ml for polyclonal and 41pg/ml for monoclonal assays. This antigenaemia was totally neutalizable with Abbot antiserum anti-p24...2 months after transplantation, all assays for p24-antigen became negative, without appearance of antibodies against HIV. Five months after transplantation our patient remains asymptomatic, renal function is excellent, p24 antigenaemia still negative and HIV antibodies still negative"
  AIDS 7:741-742.1993.1993
False-positive HIV antigens related to emergence of a 25-30kD proteins detected in organ recipients.
 Agbalika, F., Ferchal, F., Garnier, J. P., Eugene, M., Bedrossian, J. & Lagrange, P. H.
  Using two kits, the Abbot and Diagnostic Pasteur, in one study, p24 was detected transiently in 12/14 kidney recipients. Peak titres ranged from 850 to 200 000 pg/ml 7-27 days post- transplantation. Two heart and 5/7 bone marrow recipients were also positive, although the titres were lower and ranged from 140-750 pg/ml. Disappearance of p24 took longer in kidney (approximately 6 months) than in bone-marrow (approximately 4-6 weeks) recipients. According to the authors: "This may be related to differences in immunosuppression therapy". Discussing their findings they wrote: "The observation of a 25- 30kD protein [the French researchers report p24 as p25] binding to polyclonal anti-HIV human sera after immunoblots with reactive sera raises several questions. This protein could be related to a host immune response to grafts or transplants...Its early detection after transplantation might indicate the implications of immunosuppression therapy...The 25-30kD protein could therefore be compared with the p28 antigen recently described with human T-cell-related virus lymphotropic-endogenous sequence...The characterization of this 25-30kD protein may represent an important contribution to the detection of HIV-1-related endogenous retroviruses"
  AIDS 6:959-962. 1992.1992