Dissident AIDS Database

HIVIsolationDifficulty in isolating HIVGeneral
Plasma viremia in human immunodeficiency virus infection.
 Coombs RW et al.
  “Overall, plasma viremia [as measured by culture] developed during follow-up in 17 of 27 patients (63 percent) who initially did not have viremia, and was sustained in 35 (88 percent) of the 40 patients who initially had viremia”
  NEJM. 1989 Dec 14;321(24):1626-31.1989
The Efficacy of Azidothymidine (AZT) in the Treatment of Patients with AIDS and AIDS-Related Complex.
 Fischl MA et al.
  “The study population consisted of...patients with AIDS and patients with AIDS-related complex...The criteria for eligibility also included...an absolute number of [CD4 cells] less than 500 per cubic millimeter..., serum positive for antibody to HIV...HIV was isolated [by co-culture] at entry in 57% of the AZT group and 58% of the placebo group [i.e. almost half of patients with AIDS and pre-AIDS were negative by HIV by culturing techniques]”
  NEJM. 1987;317:185-191.1987
Acquired immunodeficiency syndrome in the United States: a selective review.
 Layon J, Warzynski M, Idris A.
  “In an early set of experiments, HTLV-III [HIV] was cultured from 48 subjects, including 18 or 21 patients with ARC [AIDS-related Complex, also known as pre-AIDS], 3 of 4 clinically normal mothers of children with AIDS, and [only] 26 of 72 adults and children with AIDS...”
  Critical Care Medicine. 1986;14(9):819-27.1986
Human immunodeficiency virus type I infection in homosexual men who remain seronegative for prolonged periods.
 Imagawa DT et al.
  “... claims that this shows that HIV culture-positive rate [is] 20% in high-risk, antibody-negative people”
  NEJM. 1989 Jun 1;320(22):1458-62.1989
Tumor Virus Purification using Zonal Rotors.
 Toplin, I.
  To characterise a retrovirus and its constituents one must purify the particles, that is, isolate them from any other biological constituents (this has not been done either by Montagnier or anybody else, a fact accepted by some of the best known protagonists of the HIV theory)
  Spectra , 225-235 (1973).1973
Purification and partial differentiation of the particles of murine sarcoma virus (M. MSV) according to their sedimentation rates in sucrose density gradients.
 Sinoussi, F., Mendiola, L. & Chermann, J.C.
  To characterise a retrovirus and its constituents one must purify the particles, that is, isolate them from any other biological constituents (this has not been done either by Montagnier or anybody else, a fact accepted by some of the best known protagonists of the HIV theory)
  Spectra 4, 237-243 (1973).1973
1
 Arthur, L.O., et al.
  Evidence that most "HIV" proteins are cellular proteins
  J. Virol. 69, 3117-24 (1995).1995
HIV isolation may not correlate with clinical state or immunological function of respective HIV infected patients.
 Urano H et al.
  “HIV was isolated from 32 patients (54%) of 59 [HIV+] patients examined. In the group with positive blood culture (group P), CD4+ cell count and CD4/8 were significantly lower than those in the group with negative blood culture (group N)... HIV isolation had no influence on the subsequent changes in the clinical state and immunological data.”
  Int Conf AIDS. 1994 Aug;10(2):255.1994
Viral dynamics in primary HIV-1 infection.
 Piatak M Jr et al.
  “Culturable virus in plasma was reduced to undetectable levels coincident with seroconversion in five of six patients, and was substantially reduced in the sixth. ”
  Lancet. 1993 Apr 24;341:1099.1993
Long-term symptomless HIV-1 infection in recipients of blood products from a single donor.
 Learmont J et al.
  “we identified a group of 6 subjects who had been infected [with HIV] through a single common [blood] donor...Throughout follow-up (range 6.8-10.1 years after infection), 5 of the [HIV antibody positive] recipients and the donor...remained clinically free of symptoms, with normal CD4 cell counts ... HIV-1 was isolated [via co-culture, which is not really isolation] from only 1 recipient [in other words, the only evidence of HIV was antibodies, all other measures indicated no HIV and no AIDS]”
  Lancet. 1992;340:863-7.1992
Correlation between CD4 cell counts and cellular and plasma viral load in HIV-1-seropositive individuals.
 Venet A et al.
  “HIV was isolated [using co-culture] from only 36% of plasma samples, and the isolation rate was closely related to CD4 cell counts, increasing gradually from 0% in subjects with >800 [million] CD4 cells [per liter] to 88% in those with < 100 [million] CD4 cells [per liter]...”
  AIDS. 1991;5:283-8.1991
Human Immunodeficiency Virus-infected individuals contain provirus in small numbers of peripheral mononuclear cells and at low copy numbers.
 Simmonds P et al.
  “Five patients who did not yield virus isolates [via co-culture] in a total of 14 attempts all had virus present in 1 per 10,000 or more cells, while five from whom virus was isolated in 11 of a total of 16 attempts all had virus present in 1 per 3,000 or fewer cells.”
  J Virol. 1990 Feb;64(2):864-72.1990
Recovery of human immunodeficiency virus from serum (letter).
 Michaelis BA, Levy JA.
  “Infectious virus was recovered from the serum of 20 (25.6%) of [78 randomly selected, HIV+ individuals, or whom about 30% were asymptomatic] and was generally present in low titers. Only undiluted serum (not a tenfold dilution) yielded infectious virus...In one serum sample, 25,000 infectious particles per milliliter were detected as measured by end dilution of the serum. This sample came from a clinically healthy individual with very low levels of antibody to HIV[!]. Nine of the positive serum samples came from 39 individuals whose PMCs [peripheral blood mononuclear cells] were also tested. Virus was isolated [sic] from the PMCs of approximately 50% of these individuals and one third also yielded infectious virus in their serum. Three serum samples contained infectious HIV without any virus being recovered from the individuals’ PMCs...These studies demonstrate further that not all seropositive individuals have virus recoverable from their PMCs and that isolation from serum is not a common event”
  JAMA. 1987 Mar 13;257(10):1327.1987
Isolation Frequency of Human Immunodeficiency Virus from Cerebrospinal Fluid and Blood of Patients with Varying Severity of HIV Infection.
 Chiodi F, Albert J, Olausson E, et al.
  Difficulty in finding active HIV particles in HIV + people's blood
  AIDS Res Hum Retrovirol, 1988, 4:351-3581988
Frequent Detection and Isolation of Cytopathic Retro- viruses (HTLV-II) from Patients with AIDS and at Risk for AIDS.
 Gallo RC, Salahuddin SZ, Popovic M, et al
  Difficulty in finding active HIV particles in HIV + people's blood
  Science, 1984, 224:500-5021984
Long-term symptomless HIV-1 infection in recipients of blood products from a single donor.
 Learmont J, Tindall B, Evans L, et al
  Difficulty in finding active HIV particles in HIV + people's blood
  Lancet, 1992, 340:863-867.1992
Detection, Isolation,and Continuous Production of Cytopathic Retroviruses (HTLV-II) from Patients with AIDS and Pre-AIDS.
 Popovic M, Sarngadharan MG, Read E, et al.
  Difficulty in finding active HIV particles in HIV + people's blood
  Science: 1984; 224: 497-5001984
Antibodies Reactive to Human T-Lympho-trophic Retroviruses (HTLV-II) in the Serum of Patients with AIDS.
 Sarngadharan MG, Popovic M, Bruch L, et al
  Difficulty in finding active HIV particles in HIV + people's blood
  Science, 1984, 224:506-5081984
Serological analysis of a Subgroup of Human T-Lymphotrophic Retroviruses (HTLV-II) Associated with AIDS.
 Schupbach J, Popovic M, Gilden RV, et al
  Difficulty in finding active HIV particles in HIV + people's blood
  Science, 1984, 224:503-5051984
Did Luc Montagnier discover HIV? (video interview with Professor Luc Montagnier at the Pasteur Institute July 18th 1997)
 Tahi D.
  A PCR or indeed any genomic study does not prove the detection of a retroviral genome much less that of a specific retrovirus HIV, when the RNA (cDNA) used to prove infection originated from material which contained no particles "with morphology typical of retroviruses".
  www.virusmyth.com/aids/data/dtinterviewlm.htm1998
Cell membrane vesicles are a major contaminant of gradient-enriched human immunodeficiency virus type-1 preparations.
 Gluschankof P, Mondor I, Gelderblom HR, Sattentau QJ.
  A PCR or indeed any genomic study does not prove the detection of a retroviral genome much less that of a specific retrovirus HIV, when the RNA (cDNA) used to prove infection originated from material consisting of "purified microvesicles"
  Virol. 1997;230:125-133.1997
Microvesicles are a source of contaminating cellular proteins found in purified HIV-1 preparations.
 Bess JW Jr, Gorelick RJ, Bosche WJ, Henderson LE, Arthur LO.
  "Identification and quantitation of cellular proteins associated with HIV-1 particles are complicated by the presence of non-virion-associated cellular proteins that copurify with virions. Many cellular proteins are associated with nonviral particles that bud from the surface of cells called microvesicles. Microvesicles band in sucrose gradients in a range of densities that includes the same density as retroviruses. To characterize these microvesicles, HIV-1-infected and uninfected human T-cell lines were propagated and virus and microvesicles were purified from clarified cell culture supernatants by sucrose density gradient centrifugation or centrifugation through 20% sucrose pads. Microvesicles were found to contain various proteins, including HLA DR and beta 2-M, and a substantial amount of RNA and DNA.. Although HIV-1 particles are known to contain some cellular proteins, microvesicles from HIV-1 infected H9 cells appeared to contain little or no HIV-1 gp120SU."
  Virology 1997 Mar 31;230(1):134-441997